Genetically modified mice
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Transgenic mice and rats creation

2 LINE GUARANTEE!

  • In case of one positive F0 mouse or rat with first injection,
    100 microinjection(C57BL/6J, Wistar) will be added for free of charge.
  • In case of no positive F0 mouse or rat with first injection,
    200 microinjection(C57BL/6J, Wistar) will be added for free of charge.
We will supply total support like "vector design", "Tg mice/rats creation", "Genotyping PCR", "Southern blotting", "gene expression analysis" and "breeding and stable supply". Unitech has created Tg mice more than 100 annually, and the success rate is 98%.
(* The above sentence doesn't show guarantee that we will generate Tg mice certainly.)
* Use of mouse/ rat line: C57BL/6J mouse/ Wistar rat (SD rats are available)

Tg mice creation

No. Terms Works Price Period
1 cDNA cloning Subcloning Refer to Cloning page 2weeks
2 Promoter cloning Genomic DNA cloning Refer to Cloning page
3 Expression vector constructing Vector plasmid construction 230,000yen
4 Cut out and purification of vector plasmid Cut out and purification of promoter to polyA 70,000yen 1week
5 200 injection into fertilized eggs and founder born DNA Injection into prenuclear fertilized egg and F0 born 470,000yen 8weeks
6 Genotyping PCR DNA extraction from F0 mice tail and Genotyping PCR 120,000yen 1week
7 F1 born Mating between F0 mice and WT mice 240,000yen 10weeks
8 Genotyping PCR (Germline transmission) DNA extraction from F1 mice tail and Genotyping PCR 80,000yen 1week
9 SPF check Designated bacteriological testing apparatus by complying customer's animal facility's criteria 40,000yen 2weeks
10 Delivery of mice and frozen embryo Delivery of frozen embryos or mice with dry ice by air cargo Depend on location -
Total No.4~No.9   1,020,000yen  

Tg rats creation

No. Terms Works Price Period
1 cDNA cloning Subcloning Refer to Cloning page 2weeks
2 Promoter cloning Genomic DNA cloning Refer to Cloning page
3 Expression vector construction Vector plasmid construction 230,000yen
4 Cut out and purification of vector plasmid Cut out and purification of promoter to polyA 70,000yen 1week
5 200 injection into fertilized eggs and founder born DNA Injection into prenuclear fertilized egg and F0 born 750,000yen 10weeks
6 Genotyping PCR DNA extraction from F0 rats tail and Genotyping PCR 120,000yen 1week
7 F1 born Mating between F0 rats and WT rats 430,000yen 10weeks
8 Genotyping PCR DNA extraction from F1 rats tail and Genotyping PCR 80,000yen 1week
9 SPF check Designated bacteriological testing apparatus by complying customer's animal facility's criteria 40,000yen 2weeks
10 Delivery of mice and frozen embryo Delivery of frozen embryos or rats with dry ice by air cargo Depend on location -
Total No.4~No.9   1,490,000yen  

Optional service

Works Method of detection Mouse Rat Period
Tail cut and delivery Customer themselves genotyping 30,000yen 30,000yen -
Measuring number of copy of insert DNA in mice or rats Southern blotting 240,000yen 240,000yen 1week
F2 homozygous BAC Tg mice or rats creation Genome DNA Realtime-PCR 540,000yen Depend on condition 
Frozen embryo creation 200 frozen embryos 230,000yen/per line - 1week
* SPF level of animals we generated follows Japan SPF checklist.

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BAC Tg mice and rats creation(semi KI mice and rats).

Endogenousに近い発現!

We can create BAC Tg mice or rats by modified BAC including a target promoter and an enhance region.
Your target gene will be transcribed without any effect of surrounding genomic DNA area.
(* The above sentence doesn't show guarantee that the BAC Tg mice or rats will express your inserted gene certainly.)
*Use of line: C57BL/6J mice, Wistar rats(SD rats are available)

BAC Tg mice or rats creation

No. Terms Works Mouse Rat Period
1 linearization of BAC clone Consulting and restriction site selection Please contact us Please contact us  
2 Preparation of BAC clone Purification of BAC clone 100,000yen 100,000yen 1week
3 300 Injection into fertilized eggs and founder born DNA Injection into prenuclear fertilized egg and F0 born 700,000yen 1,100,000yen 8weeks
4 Genotyping PCR DNA extraction from F0 mice or rats tail and Genotyping PCR 120,000yen 120,000yen 1week
Option: PCR genotyping for Tg Gene + both edges of BAC clone 240,000yen 240,000yen 1week
5 F1 born Mating between F0 mice or rats and WT mice or rats 240,000yen 430,000yen 10weeks
6 Genotyping PCR DNA extraction from F1 mice or rats tail and Genotyping PCR 80,000yen 80,000yen 1week
7 SPF check Designated bacteriological testing apparatus by complying customer's animal facility's criteria 40,000yen 40,000yen 2weeks
8 Delivery of mice or rats and frozen embryo Delivery of frozen embryos or mice or rats with dry ice by air cargo Depend on location Depend on location -
Total No.2~No.7   1,280,000yen 1,870,000yen  

Optional service

Works Method of detection Mouse Rat Period
Tail cut and deliver Genotyping by customer 30,000yen 30,000yen -
Measuring number of copy of insert DNA in mice or rats Southern blotting 240,000yen 240,000yen 2weeks
F2 homozygous BAC Tg mice or rats creation
Genomic DNA realtime-PCR 540,000yen 680,000yen 3months
Confirmation of expression in vivo RT-PCR, Western blotting, Immunostaining Please contact us Please contact us Please contact us
Frozen embryo creation 200 frozen embryos 230,000yen/per line - 1month
* In case of no positive F0 mouse or rat with first injection, 150 microinjection(C57BL/6J, Wistar) will be added for free of charge.

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