Genetically modified mice
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Knock in mice creation

FASTER and LOW COST!
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  • Creating Mutation KI mice: Ex)Creating disease model mice inserted the disease factor(Mutation).
  • Creating Reporter gene KI mice: Ex)Creating mice for kinetic analysis and in vivo localization of a target protein.
  • Creating cDNA KI mice: Ex)Creating mice for drug tests to generate Human Target Protein.

KI mice creation

No. Terms Works Price Period
- option cDNA cloning Refer to Cloning page  
1 KI vector design and construction Mapping of restriction site, sub-cloning from BAC and vector construction 1,340,000yen Meeting
+1month
- option Inserting Mutation 100,000yen 2weeks
2 Creation of homologous recombinant ES cells (C57BL/6J) 1st screening: ES genotyping PCR,
2nd screening: ES southern blotting,
3rd screening: Cre/Lox work test
2,000,000yen 1.5months
3 Chimeric mice born Injecting homologous recombinant ES cells. Evaluation of chimeric rate. 750,000yen 2.5months
4 F1 hetero mice born (germline transmission) PCR genotyping 750,000yen 3months
- Option Remove neo cassette from F1 hetero mice 350,000yen 3months
5 SPF check Designated bacteriological testing apparatus by complying customer's animal facility's criteria 40,000yen 0.5months
6 Delivery of mice and frozen embryo Delivery of frozen embryos or mice with dry ice by air cargo Depend on location  
  Subtotal 1~5 4,880,000yen  

Option service

Works Price Period
Remove Cre and Genotyping PCR 300,000yen 3months
Creation of homozygous mice including genotyping PCR 540,000yen 3months
Preparation of frozen embryos 230,000yen/per line 1week
* The above periods shows normal speed of our work.
* SPF level of animals we generate follows main Japan SPF checklist.

Knock out mice creation

SPEEDY work!

Null KO mice creation

No. Terms Works Price Period
1 Targeting vector design and construction Mapping of vector 950,000yen 0.5months
Vector construction
- Option: Vector construction for Lox-Neo cassette. 150,000yen  
2 Creation of homologous recombinant ES cells (C57BL/6J) 1st screening: ES genotyping PCR.
2nd screening: ES southern blotting.
1,700,000yen 1.5months
3 Chimeric mice born Injecting homologous recombinant ES cells, Evaluation of chimeric rate. 750,000yen 2.5months
4 F1 hetero mice born (germline transmission) PCR genotyping 750,000yen 3months
5 SPF check Designated bacteriological testing apparatus by complying customer's animal facility's criteria 40,000yen 0.5months
6 Delivery of mice and frozen embryo Delivery of frozen embryos or mice with dry ice by air cargo Depend on location  
  Subtotal 1~5 4,190,000yen  

Conditional KO mice creation

No. Terms Works Price Period
1 Targeting vector design and construction Mapping of restriction site, sub-cloning from BAC clone and vector construction 1,150,000yen 1month
- Option: Vector construction for special request Please contact us Please contact us
2 Creation of homologous recombinant ES cells (C57BL/6J) 1st screening: ES genotyping PCR
2nd screening: ES southern blotting
3rd screening: Cre/Lox work test
2,000,000yen 1.5months
3 Chimeric mice born Injecting homologous recombinant ES cells, Evaluation of chimeric rate. 750,000yen 2.5months
4 F1 hetero mice born (germline transmission) Including germline transmission 750,000yen 3months
- Option: Remove neo cassette from F1 hetero mice 350,000yen 3months
5 SPF check Designated bacteriological testing apparatus by complying customer's animal facility's criteria 40,000yen 0.5months
6 Delivery of mice and frozen embryo Delivery of frozen embryos with dry ice by air cargo Depend on location  
  Subtotal 1~5 4,690,000yen  
* The above periods shows normal speed of our work.
* SPF level of animals we generate follows main Japan SPF checklist.

Option service

Works Price Period
Remove Cre and Genotyping PCR 300,000yen 2months
Remove FLP and Genotyping PCR 300,000yen 2months
Crossing with tissue specific cre mice and genotyping PCR 350,000yen 2months
Creation of homozygous mice including genotyping PCR 540,000yen 3months
Preparation of frozen embryos 230,000yen/per line 1week

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