Recombinant protein expression and purification
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Recombinant protein expression and purification with mammalian cells

Making recombinant protein with transient expression system of mammalian cells.

Order now!! Vector construction~ high density culture and more!!
Good enough quantity by calculating a result of trial culture and purification.
This system is not only for expression of high molecular weight protein and glycoprotein, but also reconstruction of multimeric protein.
Speedy work!
* Standard of yield: 0.1mg/L~
Terms Price Period
Expression vector construction 150,000yen 2weeks
Requirement study for transfection
Requirement study for expression
(Qualitative examination)
350,000yen 1week
Small scale culture (Including SDS-PAGE and Western Blotting)
*The scale: 250ml
200,000yen 2weeks
Affinity tag purification 350,000yen 1week
Optional prurification:
Gel filtration purification(HPLC) Ion exchange purification(HPLC)
250,000yen
150,000yen
1week
1week
Submission of interim report:
results for SDS-PAGE, Western blotting and yield of the protein
For free 2days
Large scale culture 300,000yen/L -
Large scale purification (extraction from cells body and salting-out) Please contact us -

Establish cell line of stable cells expressing recombinant protein

For expression cell transmembrane or secretory protein!!

Stable cells lead expression and purification of both transmembrane and secretory protein without repeating transfection.
We can make stable cells with serum free medium.
The way of screening: Western blotting, RT-PCR and substrate-enzyme active etc.
Host cells: adhesion system CHO, suspension system CHO, adhesion system HEK293, adhesion system COS7, etc.
* Standard of yeild: 0.1 mg/L~
Terms Price Period
Expression vector work test 300,000yen 1month
Making stable expressing cells for secretory protein
(Transmembrane protein)
950,000yen
(+500,000yen)
3months
(+1month)
High density culture of stable cells 100,000yen/L  
Protein purification from culture medium: affinity column, ion exchange column and gel filtration column Please contact us  
Option: naturalization from plate culture to high density one. Please contact us  

Culture of yeast cells・Purification of protein

In yeast cells, relatively high molecular weight soluble protein can be expressed easier than in E.coli.
You can expect target protein expresses with natural holding and it can be prevented from degradation by protease and heat shock protein.
Minimum risk of cotaminatnt like virus and oncogenes in product.
Order with neither minimal scale nor maximal one.
We can seek the best conditions of expression, culture and purification.
All protocols belong to you.
* Standard of yield: 1~10mg/L
Terms Price Period
Expression vector construction 150,000yen 2weeks
Requirement study for expression with dish culture
(Qualitative examination)
350,000yen 2weeks
Small scale culture(Including SDS-PAGE and Western Blotting)
*The scale: 250ml
350,000yen 1week
Affinity tag purification 250,000yen 2weeks
Optional purification:
Gel filtration purification(HPLC)
Ion exchange purification(HPLC)
250,000yen
150,000yen
1week
1week
Submission of interim report:
results for SDS-PAGE, Western blotting and yield of the protein
For free 2days
Large scale culture basic cost:100,000yen
+80,000yen/L
-
Large scale purification (including extraction from yeast body and salting-out) Please contact us -

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