Recombinant protein expression and purification
TOP > 【Recombinant protein expression and purification】 - Expression vector construction / With E.coli / With insect cell by means of baculovirus

Recombinant protein expression and purification

MANY ACHIVEMENTS!

・Consult us about vector design~ construction~ a large scale of culture and purification.
・Meet all your needs.
・Suggest a test plan which system is suitable to your purpose of protein from gene information (Ex. Accession Number of Gene Bank or sequences).
・Compare 4 expression systems, "E.coli", "Yeast", "Insect cell" and "mammalian cell" at the same time.
・All protocols belong to you
Ask for UNITECH Co., Ltd.
COUNT ON US!
For your experiment and special usage propose

Expression vector construction for protein

You can get optimal expression vectors within several weeks.

We have already dealt with more than several thousand orders so far!
Our experts have never failed vector construction.
Suggest the best plan. Choose best promoter and enhancer for your purpose and host cell.
Design of expression vector for "tissue specific", "conditional", "drug-induced" and "genome"
1)We can design vector including sequence of Kozak, Signal, Tag, Reporter and more for your purpose
2)We can insert cDNA with restriction enzyme site and PCR precisely.
3)We are certain to confirm inserted DNA sequence precisely.
  Price Delivery period
Vector Construction 150,000yen ≧5days

Recombinant protein expression and purification with E.coli

・We can optimize DNA sequence for E.coli by codon usage.
・We can pursue optimal conditions of induction and culture.
・We can refold expressed protein of inclusion body.
Available: 100ml~100L culture and purification.
* Standard of yield: 10~100mg/L
Terms Price Period
Expression vector construction 150,000yen 2weeks
Requirement study for expression with dish culture (Qualitative examination) 200,000yen 2weeks
Small scale culture (check of expression level with SDS-PAGE and western blotting)
*The scale: 250ml
100,000yen 1week
Affinity tag purification 300,000yen 1week
Optional prurification:
Gel filtration purification(HPLC)
Ion exchange purification(HPLC)
250,000yen
150,000yen
1week
1week
Submission of interim report:
results for SDS-PAGE, Western blotting and yield of the protein
For free 2days
Large scale culture Basic cost: 80,000yen
+20,000yen/L
-
Large scale purification (including extraction from E.coli body and salting-out) Please contact us -
[*Reference] Research agenda:(1)Temperature(3 points), (2)Culture time(3 points), (3)Two conditions of IPTG induction

Culture of insect cells/ Expression and purification with insect cells expression systems(baculovirus)

Make you satisfied with MORE RELIABLE RESULTS!

If you need massive protein having similar modification of mammalian, order NOW!
If you need correct folding protein , order NOW!
・Baculovirus is available in more than P1 level lab since it can not be infected with vertebrate.
・From trial scale(250ml) to large scale(300L) culture is acceptable
・There is already a pipeline protein made in insect cells with baculovirus, which is on phase III.
* Standard of yield: 10~100mg/L
Terms Price Period
Making baculovirus and titer check 450,000yen 6weeks
Requirement study for expression (Qualitative examination) 250,000yen 2weeks
Small scale culture (Including SDS-PAGE and Western Blotting)
*The scale: 250ml
100,000yen 1week
Affinity tag purification 300,000yen 1week
Optional prurification:
Gel filtration purification(HPLC)
Ion exchange purification(HPLC)
250,000yen
150,000yen
1week
1week
Submission of interim report:
results for SDS-PAGE, Western blotting and yield of the protein
For free 2days
Large scale culture 150,000yen/L -
Large scale purification (including extraction from insect body and salting-out) Please contact us -
* For more information, please contact us.

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